Template Switch Oligo
Template Switch Oligo - Use this set of oligonucleotides for template switching cdna synthesis and preamplification. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. The resulting cdna can be amplified by pcr or serve as. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end.
We recommend a tso with rgrgrg at the 3′ end. However, if a large fraction of the library contains a portion. The resulting cdna can be amplified by pcr or serve as. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor.
Template Switch Oligo Printable Word Searches
Template Switch Oligo Printable Word Searches
Template Switch Oligo
Template Switch Oligo
Template Switch Oligo Printable Word Searches
We recommend a tso with rgrgrg at the 3′ end. The resulting cdna can be amplified by pcr or serve as. The tso adds a common 5'. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end.
The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2.
In Conjunction With A Template Switching Oligo (Tso), Cdna Is Synthesized With A Known Sequence Of Choice Attached To The 3′ End.
Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. The resulting cdna can be amplified by pcr or serve as. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. The tso adds a common 5'.
In General, Different Tsos Can Be Used Including Tsos With 5′ Modifications, Such As Biotin, Isomeric Bases Or Abasic Spacers.
However, if a large fraction of the library contains a portion. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. We recommend a tso with rgrgrg at the 3′ end. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2.